What
is it?
Delila-Genome is a system for identification, visualization and
analysis of protein binding sites in complete genome sequences.
What is the basis for
identification of the binding sites?
Shannon Information theory. Read about the application of information
theory to molecular biology at
Dr.
Tom Schneider's page.
Any dependencies?
This system uses the
Delila
system tools for the identification of potential sites over the
whole genome. From these potential sites, our tools select and visualize
those sites which are within or adjacent to expressed loci in the human
genome sequence. The visualization tools take advantage of the
UCSC
genome browser.
What are the components of Delila-Genome?
The system has three main components: (A) The front-end (B)The server
(C)Post scan tools
Fig.
1 gives a detailed architecture of the sytem.
Fig.
2 shows screenshots of the HTML page generated by the system's
visualization tool (genvis) and
other websites linked to this HTML page. This page was generated from predictedbinding
sites on chromosome 1 for the transcription factor PXR/RXRα.
Fig.
3 shows screenshots of scandiff
(a post scan tool to compare the results of genome scans with multiple
versions of the weight matrix) results around the gene CYP3A4 for
the PXR/RXRα transcription
factor.
How can this system be utilized?
Possible identification of transcription factor binding sites and mRNA
splicing factors sites.
Prediction of alternate splicing sites.
Discovery
of novel regulatory target genes.
What types of binding sites were
scanned?
We have used this system to scan the human genome for transcription
factors like PXR/RXRα, AHR, NF-kB p50/p65 and mRNA
splicing factors like donors, acceptors, and SC35.
What software was used in building
this system?
Server - Perl, Pascal, Bash
Front end - Java, HTML
On what systems will this software
run on?
The
server should work on all major Linux versions but it can be
ported to Solaris with little or no modifcations. The front-end should
work with all systems supporting Java (1.2 or higher versions).